摘要 目的:明确附着在牙根表面的牙骨质基质提取物具有促进牙周膜细胞附着的功能。方法:采集健康的牙龈组织和牙周膜,体外培养牙龈成纤维细胞和牙周膜成纤维细胞。从因正畸需拔除的健康牙体表面获得牙骨质基质提取物,分别观察不同作用时间、不同浓度的牙骨质基质提取物对牙龈成纤维细胞和牙周膜成纤维细胞在牙体表面附着的影响。结果:牙龈成纤维细胞、牙周膜成纤维细胞均对牙骨质基质提取物有浓度和时间依赖性，最佳作用时间为2小时，最合适浓度为10 μg/ml。且牙骨质基质提取物促牙周膜成纤维细胞附着的作用高于对牙龈成纤维细胞的作用。结论:牙骨质基质提取物能够促进牙周膜细胞在牙根表面上的附着。
Extract of Cemental Matrix Enhance Periodontal Cells Bind to the Root Surface
Sui Wen, Hong Yonglong, Xiao Minzhen
College of Stomatology, the Fourth Military Medical University
Abstract Objective: To investigate whether the extract of cemental matrix can enhance periodontal cells binding to the root surfaces.Methods: Healthy human gingiva and periodontal ligament were acquired from patients, then gingival fibroblasts and periodontal cells were cultured in vitro. On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment. Finally, the effects of cementum matrix with different concentration and different time on the attachment of gingival fibroblast and periodontal cells to root surface were observed.Results: The extract of cemental matrix could enhance the initial attachment of human gingival fibroblasts (HGF) and human periodontal cells (HPC) to the surface of root, and such effects were strengthened with increasing density and time. The optimal concentration of extract of cell attachment 10μg/ml, and the attachment was also proportional to the incubation time,
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